Phytochemical study, nutritive value, antioxidant and anti-inflammatory activities of phenolic extracts from desert plant Calligonum comosum L'Hér.
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Abstract
The aim of this study is to the estimation of the nutritive values, phytochemical study and the antioxidant and anti-inflammatory activities of ethyl acetate, tannins and crudes extracts from the aerial part of Calligonum comosum L’Hér. Which grow in South East of Algeria. According to the results obtained from the estimation of nutritive value, the plant is rich in proteins and lipids and very poor in carbohydrates. The results also showed a difference in the content of polyphenols and flavonoids. The crude extracts were high in polyphenols content in the ethanol extract 170.74±5.8 mg EAG/g Extract, and the best value of flavonoids in methanol extract 28.22±0.25 mg QE/g Extract. Chromatographic analysis by HPLC of methanolic extract has identified four phenolic compounds out of 38 peaks as: Gallic acid, Chlorogenic acid, Caffeic acid and Vanillic acid. The antioxidant activity was evaluated by three tests, the results of the DPPH• test, showed that methanol extract had the best scavenging than the other extracts (IC50: 2.09±0.02 μg/ml), But, in the hemolysis test, the values of the percentage of hemolysis were approximately the same at concentration 1 mg/ml between the extracts and standard used. In the reducing power test, its results showed that the tannins extract had the highest reducing power 79.17±5.22 μg/ml at Abs700nm=0.5. As for anti-inflammatory activity, the methanol and flavonoids phase ethyl acetate extracts showed the best inhibitory effect against protein denaturation compared to other extracts. The effects were estimated 3.14mg E Diclofenac/mg Extract.
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. Anoop MV, Bindu AR. In-vitro Anti-inflammatory Activity Studies on Syzygium zeylanicum (L.) DC Leaves. International Journal of Pharma Research & Review. 2015, 4;8:18-27.
. Bekkara F, Jay M, Viricel MR, Rome S. Distribution of phenolic compounds within seed and seedlings of tow Vicia faba vs differing in their seed tannin content, and study of their seed and root phenolic exudation. Plant and Soil. 1998, 203:27-36. DOI: https://doi.org/10.1023/A:1004365913726
. Binghua T, Shengji L, Wenjun C, Xinghuai S. The Association of Oxidative Stress Status with Open-Angle Glaucoma and Exfoliation Glaucoma: A Systematic Review and Meta-Analysis. Journal of Ophthalmology. 2019, Article ID 1803619:14 pages. DOI: https://doi.org/10.1155/2019/1803619
. Bogusław B, Sylwia N. Hydrophilic interaction liquid chromatography (HILIC)—a powerful separation technique. Analytical and Bioanalytical Chemistry. 2012, 402;1:231-47. DOI: https://doi.org/10.1007/s00216-011-5308-5
. Bouzid W, Yahia M, Abdeddaim M, Aberkane C, Ayachi A. Evaluation de l’Activité antioxydante et Antimicrobienne des extraits de l’Aubepine monogyne. Lebanese Science Journal. 2010, 12;1:59-69.
. Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology. 1995, 28:25-30. DOI: https://doi.org/10.1016/S0023-6438(95)80008-5
. Carocho M, Ferreira ICFR. The role of phenolic compounds in the fight against cancer–a review. Anti-Cancer Agents in Medicinal Chemistry. 2013, 13;8:1236-58. DOI: https://doi.org/10.2174/18715206113139990301
. Cheruth AJ, Alnaqbi KM, El-kaabi A, Odeh OW, Kandhan K, Maqsood S, Kurup SS, Sakkir S. In vitro antioxidant activities and screening of phytochemicals from methanolic and ethyl acetate extracts of Calligonum comosum L’Hér. Oriental Pharmacy and Experimental Medicine. 2016, 16:209-15. DOI: https://doi.org/10.1007/s13596-016-0232-z
. Chirisa, E., Mukanganyama, S., (2016): Evaluation of in Vitro Anti-inflammatory and Antioxidant Activity of Selected Zimbabwean Plant Extracts. Journal of Herbs, Spices & Medicinal Plants, 22(2): 157-172. DOI: https://doi.org/10.1080/10496475.2015.1134745
. Chouikh A, Adjal EH, Mekki M, Hemmami H, Feriani A, Rebiai A, Zaater A, Chefrour A. Comparison of ultra-sound and maceration extraction methods of phenolics contents and antioxidant activities of Saharian medicinal plant Calligonum comosum L’Hér. Journal of Materials and Environmental Science. 2016, 7;6:2235-9.
. Chouikh A, Alia F, Neffar S, Rebiai A, Adjal E, Chefrour A. Evaluation of phenolic contents (quantitative and qualitative) and antioxidant activities in different physiological phases of Genista saharae Coss. & Dur. growing in the Sahara of Algeria. Analele Universitatii din Oradea Fascicula Biologie. 2018,25;2:115-21.
. Chouikh A, Mekki M, Adjal EH. Effects of extraction methods on antibacterial activity of different extracts of Calligonum comosum L’Hér. growing in Sahara Algerian. International Journal of Recent Scientific Research. 2015, 6;4:3534-6.
. Dias DA, Urban S, Roessner U. A Historical Overview of Natural Products in Drug Discovery. Metabolites. 2012, 2;2:303-36. DOI: https://doi.org/10.3390/metabo2020303
. Dolci A, Panteghini M. Harmonization of automated hemolysis index assessment and use: Is it possible?. Clinica Chimica Acta. 2014, 432:38-43. DOI: https://doi.org/10.1016/j.cca.2013.10.012
. DuBois MK, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Analytical Chemistry. 1956, 28;3:350-6. DOI: https://doi.org/10.1021/ac60111a017
. Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology. 2005, 4;7:685-8. DOI: https://doi.org/10.5897/AJB2005.000-3127
. Elias G, Rao MN. Inhibition of albumin denaturation and anti-inflammatory activity of dehydrozingerone and its analogs. Indian Journal of Experimental Biology. 1988, 26;7:540-2.
. Ghedadba N, Hambaba L, Ayachi MC, Aberkane H, Bousselsela SM, Oueld-Moukhtar SM. Polyphénols totaux, activités antioxydant et antimicrobienne des feuilles de Marrubium deserti de Noé. Phytothérapie. 2015, 13:118-29. DOI: https://doi.org/10.1007/s10298-015-0944-4
. Goldsworthy AC, Mordue W, Guthkelch J. Studies on insect adipokinetic hormone. General and Comparative Endocrinology. 1972, 18;3:545-51. DOI: https://doi.org/10.1016/0016-6480(72)90034-2
. Hammar KA, Carson CF, Riley TV. Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology. 1999, 86:985-90. DOI: https://doi.org/10.1046/j.1365-2672.1999.00780.x
. Jeong SM, Kim SY, Kim DR, Jo SC, Nam KC, Ahn DU, Lee SC. Effects of heat treatment on the antioxidant activity of extracts from Citrus peels. Journal of Agricultural and Food Chemistry. 2004, 52:3389-93. DOI: https://doi.org/10.1021/jf049899k
. Khelef Y, Chouikh A, Rebiai A, Neffar S, Chefrour A, Adjal EH, Alia F. Biochemical, quantitative and qualitative phenolic compounds, anti-free radical's activities of Calligonum comosum collected from different sites in the Algerian Desert. Biharean Biologist. 2019, 13;2:71-6.
. Konkon NG, Simaga D, Adjoungova ALN, Guessan KE, Zirihi CN, Kone BD. Etude phytochimique de Mitragyna inermis (Willd.) O. Ktze (rubiaceae), plante a feuille antidiabetique. Pharmacopée et médecine traditionnelle africaine. 2006, 14:73-80.
. Kose K, Dogan P. Lipoperoxidation induced by hydrogen peroxide in human erythrocyte membranes. 1. Protective effect of Ginkgo biloba extract (EGb 761). Journal of International Medical Research. 1995, 23;1:1-8. DOI: https://doi.org/10.1177/030006059502300101
. Kumaran A, Karunakaran RJ. In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. LWT - Food Science and Technology. 2007, 40:344-52. DOI: https://doi.org/10.1016/j.lwt.2005.09.011
. Kurlbaum M, Högger P. Plasma protein binding of polyphenols from maritime pine bark extract (USP). Journal of Pharmaceutical and Biomedical Analysis. 2011, 54;1:127-32. DOI: https://doi.org/10.1016/j.jpba.2010.07.038
. Li J, Zhao W, Li J, Xia W, Lei L, Zhao S. Research methods to study plant secondary metabolic pathways and their applications in the analysis of the biosynthetic pathway of stilbenes from Polygonum multiflorum -A review. Plant Omics Journal. 2014, 7;3:158-65.
. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurments with the folin phenol reagent. Journal of Biological Chemistry. 1951, 193;1:265-75. DOI: https://doi.org/10.1016/S0021-9258(19)52451-6
. Luthria DL. Influence of experimental conditions on the extraction of phenolic compounds from parsley (Petroselinum crispum) flakes using a pressurized liquid extractor. Food Chemistry. 2008, 107;2:745-52. DOI: https://doi.org/10.1016/j.foodchem.2007.08.074
. Mebirouk BL, Cherif M, Boudechiche L, Sammar F. Teneurs en composés primaires et secondaires des feuilles d’arbustes fourragers de la région humide d’Algérie. Revue de Médecine Vétérinaire. 2014, 165:344-52.
. Moldovan ML, Bogdan C, Iurian S, Roman C, Oniga I, Benedec D. Phenolic content and antioxidant capacity of pomace and canes extracts of some Vitis vinifera varieties cultivated in Romania. Farmacia. 2020, 68;1:15-21. DOI: https://doi.org/10.31925/farmacia.2020.1.3
. Nour El-Din NM, Fatma AA. Effect of seasonal variation on secondary metabolites and nutritive value of Crotalaria Aegyptiaca Benth. Egyptian Journal of Desert Research. 2004, 54;9:129-47.
. Osman NI, Sidik NJ, Awal A, Adam NAM, Rezali NI. In vitro xanthine oxidase and albumin denaturation inhibition assay of Barringtonia racemosa L. and total phenolic content analysis for potential anti-inflammatory use in gouty arthritis. Journal of Intercultural Ethnopharmacolog. 2016, 5;4:343-9. DOI: https://doi.org/10.5455/jice.20160731025522
. Padmanabhan P, Jangle SN. Evaluation of in-vitro anti-inflammatory activity of herbal preparation, acombination of four medicinal plants. International Journal of Basic and Applied Medical Sciences. 2012, 2;1:109-16.
. Rice EL. Some Roles of Allelopathic Compounds in Plant Communities. Biochemical Systematics and Ecology. 1977, 5;3:201-6. DOI: https://doi.org/10.1016/0305-1978(77)90005-9
. Saague PWK, Moukette MB, Njimou JR, Biapa PCN, Tankeu FN, Moor VJA, Pieme CA, Ngogang J. Phenolic compounds from water-ethanol extracts of Tetrapleura tetraptera produced in Cameroon, as potential protectors against In vivo CCl4-induced liver injuries. The Scientific World Journal. 2019, ID 523685:10 pages. DOI: https://doi.org/10.1155/2019/5236851
. Siddhuraju P, Becker K. The antioxidant and free radical scavenging activities of processed cowpea (Vigna unguiculata (L.) Walp.) seed extracts. Food Chemistry. 2007, 101;1:10-19. DOI: https://doi.org/10.1016/j.foodchem.2006.01.004
. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture. 1965, 16;3:144-58. DOI: https://doi.org/10.5344/ajev.1965.16.3.144
. Tabuti JRS, Lye KA, Dhillion SS. Traditional herbal drugs of Bulamogi, Uganda: plants, use and administration. Journal Ethnopharmacology. 2003, 88;1:19-44. DOI: https://doi.org/10.1016/S0378-8741(03)00161-2
. Thammana M. A Review on High Performance Liquid Chromatography (HPLC). Journal of Pharmaceutical Analysis. 2016, 5;2:22-8.
. Vaquero MJR, Alberto MR, Manca de Nadra MC. Influence of phenolic compounds from wines on the growth of Listeria monocytogenes. Food Control. 2007, 18;5:587-93. DOI: https://doi.org/10.1016/j.foodcont.2006.02.005
. Williams LAD, O'Connar A, Latore L, Dennis O, Ringer S, Whittaker JA, Conrad JA, Vogler B, Rosner H, Kraus W. The in vitro anti-denaturation effects induced by natural products and nonsteroidal compounds in heat treated (immunogenic) bovine serum albumin is proposed as a screening assay for the detection of anti-inflammatory compounds, without the use of animals in the early stages of the drug discovery process. The West Indian medical journal. 2008, 57;4:327-31.
. Xiaole W, Chuqiao S, Jie Z, Guoming S, Zegeng L, Jingcheng D. Long Noncoding RNSs in the Regulation of Oxidative Stress. Oxidative Medicine and Cellular Longevity. 2019, 4:1-7. DOI: https://doi.org/10.1155/2019/1318795
. Yildirim A, Mavi A, Kara AA. Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts. Journal of Agricultural and Food Chemistry. 2001, 49;8:4083-9. DOI: https://doi.org/10.1021/jf0103572
. Zhang SY, Zheng CG, Yan XY, Tian WX. Low concentration of condensed tannins from catechu significantly inhibits fatty acid synthase and growth of MCF-7 cells. Biochemical and Biophysical Research Communications. 2008, 371;4:654-8. DOI: https://doi.org/10.1016/j.bbrc.2008.04.062
. Zheng CD, Li G, Li HQ, Xu XJ, Gao JM, Zhang AL. DPPH-scavenging activities and structure-activity relationships of phenolic compounds. Natural Product Communications. 2010, 5;11:1759-65. DOI: https://doi.org/10.1177/1934578X1000501112
. Zouari S, Dhief A, Aschi-Smiti S. Chemical Composition of Essential Oils of Calligonum comosum Cultivated at the South Eastern of Tunisia: A Comparative Study between Flowering and Fructification Stages. Journal of Essential Oil Bearing Plants. 2012, 15;2:320-7. DOI: https://doi.org/10.1080/0972060X.2012.10644054