Effects of extraction methods on total polyphenols, free radical scavenging and antibacterial activity of crude extracts of Cleome arabica L. growing in Oued Souf region

Main Article Content



This study aims to phytochemical study, antiradical scavenging and antibacterial Activity of the extracts of Cleome arabica L. from the region of Oued Souf (East South Algerian). The crud extracts two methods obtained methanolic were maceration (EM) and ultra-sound (EU). The yields were: 8.95% and 9.60%, respectively. The quantitative estimation of total polyphenols is 11.57 mg EAG/g Ex (EM) and 13.46 mg EAG/g Ex (EU). The flavonoids contents in (EM) and (EU) are respectively 5.48 and 6.19 mg EQu/g Ex. the anti-free radical activity in the extracts at (concentration 0.1mg/ml) showed a great capacity to scavenge of the DPPH• radical and the percentage of inhibition was 41% in (EM) and 60.67 % in (EU). The results of the antibacterial activity of two bacterial strains: Escherichia coli and Staphylococcus aureus, revealed that Cleome arabica L. has a significant effect on the two strains with inhibition zones variable from 0 to 12 mm for Escherichia coli and 0 to 13 mm for Staphylococcus aureus.

Article Details

How to Cite
CHOUIKH, A. (2020). Effects of extraction methods on total polyphenols, free radical scavenging and antibacterial activity of crude extracts of Cleome arabica L. growing in Oued Souf region. Algerian Journal of Biosciences, 1(1), 14–17. https://doi.org/10.57056/ajb.v1i1.23


Maire R. Flore de l’Afrique du Nord. Encyclopédie biologiques (Vol. 12). Paris: Paul Lechvalier; 1965.

Halis Y. Plant Encyclopedia in area Oued Souf: desert plants common in the Big East race. El Oued: El Walid; 2007.

Ozenda, P. Flore de Sahara. Paris: CNRS; 1991.

Gubb AS. La flore Saharienne: Un aperçu photographique. Alger: Adolphe Jourdane; 1913.

Sharaf M, Mansour RMA, Saleh NAM. Exudate flavonoids from aerial parts of four Cleome species. Biochem Syst Ecol. 1992, 20; 5: 443-448. DOI: https://doi.org/10.1016/0305-1978(92)90084-Q

Chouikh A, Mekki M, Adjal EH. Effects of Extraction Methods on Antibacterial Activity of Different Extracts of Calligonum comosum L'her. Growing In Sahara Algerian. Int J Recent Sci. 2015, 6; 4: 3534-3536.

Khosravi M, Mortazavi SA, Karimi M, Sharayie P, Armin M. Comparison of ultrasound assisted and Kelavenger extraction methods on efficiency and antioxidant properties of Fennel’s oil essence and its optimization by response surface methodology. Intl J Agri Crop Sci. 2013, 5; 21: 2521-2528.

Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Am J Enol Viticult. 1965, 16: 144-158. DOI: https://doi.org/10.5344/ajev.1965.16.3.144

Chouikh A, Adjal EH, Mekki M, Hemmami H, Feriani A, Rebiai A, Zaater A, Chefrour A. Comparison of ultra-sound and maceration extraction methods of phenolics contents and antioxidant activities of Saharian medicinal plant Calligonum comosum L’her. J Mat Env Sci. 2016, 7; 6: 2235-2239.

Brand-Williams W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. LWT-Food Sci Technol. 1995, 28; 1: 25-30. DOI: https://doi.org/10.1016/S0023-6438(95)80008-5

Treki AS, Merghem R, Dehimat L. Etude phytochimique et évaluation de l'activité antibactérienne d'une Labiée: Thymus hirtus. Sciences and Technologie. 2009, 29: 25-29.

Andrews JM. Determination of minimum inhibitory concentrations. J Antimicrob Chemother. 2001, 48; 1: 5-16. DOI: https://doi.org/10.1093/jac/48.suppl_1.5

Hayouni E, Abedrabba M, Bouix M, Hamdi M. The Effect of Solvents and Extraction Method on the Phenolic Compounds Contents and Biological Activities in Vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea L. Fruit Extract. Food Chem. 2007, 105: 1126-1134. DOI: https://doi.org/10.1016/j.foodchem.2007.02.010

Rice-Evans CA, Sampson J, Bramley PM, Holloway DE. Why do we expect carotenoids to be antioxidants in vivo?. Free Radic Res. 1997, 26; 4: 381-398. DOI: https://doi.org/10.3109/10715769709097818

Miliauskas G, Venskutonis PR, Van Beek TA. Screening of Radical Scavenging Activity of Some Medicinal and Aromatic Plant Extracts. Food Chem. 2004, 85: 231-237. DOI: https://doi.org/10.1016/j.foodchem.2003.05.007

Lambert PA. Cellular impermeability and uptake of biocides and antibiotics in Gram-positive bacteria and mycobacteria. J Appl Microbiol. 2002, 92: 46-54. DOI: https://doi.org/10.1046/j.1365-2672.92.5s1.7.x

Ghazanfar SA. Handbook of Arabian Medicinal Plants. Florida: CRC Press; 1994. DOI: https://doi.org/10.1201/b14834

Kamil M, Jayaraj AF, Ahmad F, Gunasekhar C, Samuel S, Habiballah M, Chan K. (). Pharmacognostic and phytochemical standardisation of Calligonum comosum. J Pharm Pharmacol. 2000, 52; Suppl: 262 p.