Comparative study of the antibacterial action of natural and commercial oils of Rosmarinus officinalis L
Article Sidebar
Main Article Content
Abstract
The article tries to shed light on the antibacterial power of rosemary oils to see if there is a possibility of making drugs but also to compare this power with that existing in commercial oils of rosemary and to check if these formulas really contain oils rosemary. This study consisted of the extraction of the essential oil from the studied plant followed by preliminary phytochemical tests and then by TLC analyzes to determine exactly the different compounds that characterize the species and then by the antibacterial study on the plants diluted essential oils (synthesized and commercial). Phytochemical tests have shown that essential oils contain many chemicals and active ingredients that give rosemary a wide range of indications : The TLC of the raw extract shows stains, indicating the presence of flavone, hydroxyflavone, Dihydroxyflavone, flavanones, methoxyflavones and flavonols ; they are compounds of paramount importance in the therapeutic effects enjoyed by rosemary. The bacteriological study on essential oils (extract and commercial) by the process represented on the concentrated disk of essential oils shows good antibacterial activity on the fixed stem. Essential oils extracted from rosemary from Algeria will be of some economic interest in the Algerian pharmaceutical industry. The negative test for commercial rosemary essential oil compared to the positive tests of our extract can tell us more about the counterfeit that characterizes this area. The study confirms the interest we must bring to this plant, not only for its characteristics in the perfume industry, but especially for its undisputed medical virtues.
Article Details
References
. AFNOR. (1986). Recueil des Normes Françaises « huiles essentielles », AFNOR. Paris. 57 p.
. Andrews J.M. (2001). The Development of the BSAC standardized method of disc diffusion testing. J. Antimic. Chemo., 48 (1) : 29-42. DOI: https://doi.org/10.1093/jac/48.suppl_1.29
. Arnold N. (1985). Contribution à la connaissance ethnobotanique et médicinale de la flore de Chypre., Université René Descarte de Paris, 3, pp. 1203-1210.
. Banerjee S., Banerjee R.P., Pradhan N.K. (2014). A Comparative Study on Antimicrobial Activity of Leaf Extract of Five Medicinal Plants and Commonly Used Antibiotics, American Journal of Phytomedicine and Clinical Therapeutics; 2(6): 788–795. 43.
. Belkhiri F.Z. (2015). Etude de l’activité antibactérienne des huiles essentielles de Rosmarinus officinalis L. Master II Génie des Procédés, Univ. Mohamed Khider-Biskra. 51 p.
. Benikhlef A (2014). Comparaison entre les huiles essentielles et leurs effets antibactériens sur Rosmarinus officinalis de la région de Bechar et Ouargla. Mémoire Pour l'Obtention du diplôme de master en Agronomie Option : Amélioration de la Production Végétale. Université Abor Belkaid-Tlemcen Faculté des Scienla nature et de la vie.
. Burt S. (2004). Essential oils: Their antibacterial properties and potential applications in foods: A review. Int. J. Food Microbiol. 94: 223-253. DOI: https://doi.org/10.1016/j.ijfoodmicro.2004.03.022
. Boutabia, L., Telailia, S., Bouguetof, I., Guenadil, F & Chefrour, A. (2016). Composition chimique et activité antibactérienne des huiles essentielles de Rosmarinus officinalis L. De la région de Hammamet (Tébessa-Algérie). Bulletin de la Societe Royale des Sciences de Liege. 85. 174-189. DOI: https://doi.org/10.25518/0037-9565.6050
. Bruneton J. (1999). Pharmacognosie, Phytochimie, Plantes médicinales, 3ème édition. Paris, Editions TEC & DOC Lavoisier. 1120-1127.
. Chao S.C., Young D.G. & Oberg G.J. (2000). Screening for Inhibitory Actvity of Essential oils on Selected Bacteria, Fungi and Viruses. J. Essent. Oil Res., 12: 639-649. DOI: https://doi.org/10.1080/10412905.2000.9712177
. Clevenger J. (1928). J. Am. Pharm. Assoc. 17 345. DOI: https://doi.org/10.2307/804043
. De Billerbeck V.G. (2007). Huiles essentielles et bactéries résistantes aux antibiotiques. Phytothérapie, 5 (5) : 249-253 DOI: https://doi.org/10.1007/s10298-007-0265-z
. Deghbouche A., Bellaa S. (2013). Étude des huiles essentielles du romarin et leurs effets anti bactériens. Mémoire pour l’obtention du diplôme de master. Université Abbès laghrour Khenchela.
. Ergun F. (2008). Antimicrobial activity of flavonoids against extended-spectrum lactamase (ESL)-producing Klebsilla pneumonia. Trop. J. pharme. Res. 7 (4), 1151- 1157 DOI: https://doi.org/10.4314/tjpr.v7i4.14701
. Guy D. (1964). Organisation et classification des pantes vasculaires, cours de botanique générale. Ed. SEDES. 435p
. Hermal C. (1993). Activité bactériostatique de sept émulsions d'huiles essentielles et de deux associations d'émulsions d'huiles essentielles. Thèse, Faculté de pharmacie, Université Montpellier I. 87 p.
. Lehmann H. (2013). Le médicament à base de plantes en Europe. Statut, Enregistrement, contrôle. Thèse pour l’obtention du grade de docteur de Strasbourg. Vol. I., 229 p
. Lewis Y.S., Krishnamoorthy N., Shivashanker S., Natajan C.P. (1977). Quality studies on cinnamons marketed in India I.S.I. Bulletin. 29(12/12) : 307-312.
. Martini A. (2004). Seven flavonoïds with antibacterial activity isolated from Combretum erythrophyllum. J. Ethnopharmacol . 93(23): 207-12. DOI: https://doi.org/10.1016/j.jep.2004.02.030
. Moreira M.R., Ponce A.G., de Valle C.E. & Roura S.I. (2005). Inhibitory parameters of essential oils to reduce a food borne pathogen. Lebensmittel-Wissenschaft und -Technologie-LWT, 38 : 565-570 DOI: https://doi.org/10.1016/j.lwt.2004.07.012
. Osuagwu G.G.E., Ihenwosu A.O. (2014). Phytochemical Composition and Antimicrobial Activity of the Leaves of Alchornea cordifolia (Schum and Thonn), Sanseviera liberica (Gerand Labr) and Uvaria chamae (P. Beauv), American Journal of Phytomedicine and Clinical Therapeutics; 2(1): 001– 012
. Ouibrahim A., Tlili Ait Kaki Y., Bennadja S., Amrouni S., Djahoudi A.G., Djebar M.R. (2013). Evaluation of antibacterial activity of Laurus nobilis L., Rosmarinus officinalis L. and Ocimum basilicum L. from Northeast of Algeria. African Journal of Microbiology Research, 7 (42) : 4968-4973. DOI: https://doi.org/10.5897/AJMR2012.2390
. Santoyo S., Cavero S., Jaime L., Ibañez E., Señoráns F.J., Reglero G. (2005). Chemical composition and antimicrobial activity of Rosmarinus officinalis L. essential oil obtained via supercritical fluid extraction. J. Food Prot., 68 (4): 790-795 DOI: https://doi.org/10.4315/0362-028X-68.4.790
. Wilkinson J.M. (2006). Methods for testing the antimicrobial activity of extracts. Chapiter VIII.pp.157-165. In Ahmad I., Aqil F. and Owais M. Modern Phytomedicine : Turning Medicinal Plants into Drugs. Ed. WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim, 405 p DOI: https://doi.org/10.1002/9783527609987.ch8
. Zeghad N. (2009). Etude du contenu polyphénolique de deux plantes médicinales d’intérêt économique (Thymus vulgaris, Rosmarinus officinalis) et évaluation de leur activité antibactérienne. Mémoire en vue de l’obtention du diplôme de magister (Ecole doctorale) Option : Biotechnologie végétale. Université Mentouri Constantine Faculté des sciences de la nature et de la vie Département de biologie végétale et écologie.