Potential hepatoprotective effect of the crude extract of Lepidium sativum on an experimental model of hepatotoxicity
Article Sidebar
Main Article Content
Abstract
To investigate the effect of the high paracetamol doses on liver damage, we developed an experimental model of hepatotoxicity induced by paracetamol. In our study, we were interested in the evaluation of the potential hepatoprotective effect of the methanolic extract of Lepidium sativum (L.sativum ME). The latter, was prepared by maceration in methanol and then analyzed qualitatively, using staining and precipitation methods. Mice were randomly allocated into four groups: Ctrl, Paracetamol, ME (200mg/Kg) /Paracetamol and Paracetamol groups /ME (400 mg/Kg). Mice were euthanized and associated indications were investigated to evaluate the histological changes in the liver of the different groups as well as the degree of liver damage caused by paracetamol with and without treatment. With regard to our results, we have noted with interest a hepatoprotective effect of L.sativum ME. Indeed, our results indicate an improvement of the anatomopathological architecture of the hepatic sections following treatment with L.sativum ME. These observations could promote a potential target for the treatment of hepatic injury.
Article Details
References
Subramoniam, A.; Pushpangadan, P. Development of phytomedicine for liver diseases. Indian journal of Pharmacology 1999, 31(3): 166–175.
Adewusi, EA.; Afolayan, AJ. A review of natural products with hepatoprotective activity. Journal of Medicinal Plants Research 2010, 4(13): 1318–1334. https://doi.org/10.5897/JMPR09.472
Hinson, JA.; Roberts, DW.; James, LP. Mechanisms of Acetaminophen-Induced Liver Necrosis. Handbook of experimental pharmacology 2010, 196: 369–405. https://doi.org/10.1007/978-3-642-00663-0_12
Gonçalvesa, DF.; de Carvalho, NR.; Leite, MB.; Courtes, AA; Hartmann, DD.; Stefanello, ST.; Silva Ingrid, Kd.; Franco,; Jéferson L.; Soares Félix, AA.; Dalla, C.; Cristiane, L. Caffeine and acetaminophen association: Effects on mitochondrial bioenergetics. Life Sciences 2018, 193: 234-241. https://doi.org/10.1016/j.lfs.2017.10.039
Mc Gill, MR.; Jaeschke, H. Metabolism and disposition of acetaminophen: recent advances in relation to hepatotoxicity and diagnosis. Pharmaceutical research 2013, 30(9): 2174-2187. https://doi.org/10.1007/s11095-013-1007-6
Mazraati, P.; Minaiyan, M. Hepatoprotective Effect of Metadoxine on Acetaminophen-induced Liver Toxicity in Mice (2018). Advanced biomedical research 2018, 7(1): 67. https://doi.org/10.4103/abr.abr_142_17
Adeleke, OV.; Adefegha, SA.; Oboh, G. Mechanisms of medicinal plants in the treatment of diabetic wound. Asian Pacific Journal of Tropical Biomedicine 2023, 13(6): 233-241.doi: 10.4103/2221-1691.378597
Al-Yahya, MA.; Mossa, JS.; Ageel, AM.; Rafatullah, S. Pharmacological and safety evaluation studies on Lepidium sativum L. Seeds. Phytomedicine 1994, 1(2) : 155–159. https://doi.org/10.1016/S0944-7113(11)80035-8
Sowjanya, KR.; Vishweswar, RV.; Bushra, F.; Kapila, M.; Srilakshmi, M. In vitro anti-inflammatory activity of ultra soni assisted methanol extract of Lepidium sativum linn seeds. Int J Pharm Dev Technol 2013, 3:63–5.
Indumathy, R.; Aruna, A. Cytotoxic potential of various extracts of Lepidium sativum (Linn.): An in-vitro evaluation. Int J Pharm Pharm Sci 2015, 2(5):1–5.
Al-Sheddi, ES.; Farshori, NN.; Al-Oqail, MM.; Musarrat, J.; Al-Khedhairy, AA.; Siddiqui, MA. Protective effect of Lepidium sativum seed extract against hydrogen peroxide-induced cytotoxicity and oxidative stress in human liver cells (HepG2). Pharmaceutical biology 2016, 54(2): 314-321. https://doi.org/10.3109/13880209.2015.1035795
Raish, M.; Ahmad, A.; Alkharfy, KM.; Ahamad, SR.; Mohsin, K.; Al-Jenoobi, FI.; Ansari, MA. Hepatoprotective activity of Lepidium sativum seeds against D-galactosamine/lipopolysaccharide induced hepatotoxicity in animal model. BMC Complementary and Alternative Medicine 2016, 16(1): 501. https://doi.org/10.1186/s12906-016-1483-4
Alqahtani, FY; Aleanizy, FS; Mahmoud, AZ.; Farshori, NN.; Alfaraj, R.; Al-Sheddi, ES. Chemical composition and antimicrobial, antioxidant, and anti-inflammatory activities of Lepidium sativum seed oil. Saudi journal of biological sciences 2019, 26(5):1089–1092. https://doi.org/10.1016/j.sjbs.2018.05.007
Attia, ES.; Amer, AH.; Hasanein, MA. The hypoglycemic and antioxidant activities of garden cress (Lepidium sativum L.) seed on alloxan-induced diabetic male rats. Natural product research 2019, 33(6):901–905. https://doi.org/10.1080/14786419.2017.1413564
Hadj Rabia, S.; Debib, A.; Eddaikra, A.; Aberkane-Mchebbek, L.; Nouri, R.; Benmoussa, F.; Mokhtari, A.; Medjber, S.; Mansouri, B.; Messaoudi, M. Impact of gamma irradiation on phytochemical composition, and biological activities of Lepidium sativum seeds extract. Radiochimica Acta 2024, 112(5): 351-362. https://doi.org/10.1515/ract-2023-0260
Tufail, T.; Khan, T.; Bader, Ul.; Ain, H.; Morya, S.; Shah, MA. Garden cress seeds: a review on nutritional composition, therapeutic potential, and industrial utilization. Food Science & Nutrition 2024, 12(6):3834-3848. https://doi.org/10.1002/fsn3.4096
Trease GE, Evans WC. A textbook of Pharmacogno.sy (13th edition) Bacilluere Tinal Ltd, London; 1989.
Harbone JB. Methods of extraction and isolation in phytochemical methods, 3rd Edition, Chapman and Hall, London; 1998, p60-66.
Organisation de développement économique et culturel (OCDE). Lignes directrices de l'OCDE pour les essais de produits chimiques (2008). Toxicité orale aiguë - Méthode de l'ajustement des doses. https://read.oecd-ilibrary.org/environment/essai-n-425-toxicite-aigue-par-voie-orale-methode-de-l-ajustement-des-doses_9789264071056-fr#page1
Labsi, M. ; Soufli, I. ; Touil-Boukoffa, C. ; Amir, ZC. Hepatic inflammation and liver fibrogenesis: a potential target for the treatment of cystic echinococcosis-associated hepatic injury. Acta tropica 2021, 226: 106265. https://doi.org/10.1016/j.actatropica.2021.106265
Labsi, M.; Khelifi, L.; Mezioug, D; Soufli, I.; Touil-Boukoffa, C. Antihydatic and immunomodulatory effects of Punica granatum peel aqueous extract in a murine model of echinococcosis. Asian Pacific journal of tropical medicine 2016, 9(3): 211-20. https://doi.org/10.1016/j.apjtm.2016.01.038
Bouterfas, K.; Mehdadi, Z.; Elaoufi, M.; Latreche, A.; Benchiha, W. Antioxidant activity and total phenolic and flavonoids content variations of leaves extracts of white Horehound (Marrubium vulgare Linné) from three geographical origins. Annales pharmaceutiques francaises 2016, 74(6): 453-462. https://doi.org/10.1016/j.pharma.2016.07.002
Khattak, KF.; Simpson, TJ.; Ihasnullah, I. Effect of gamma irradiation on the extraction yield, total phenolic content and free radical-scavenging activity of Nigella sativa seed. Food chemistry 2008, 110(4): 967-972. https://doi.org/10.1016/j.foodchem.2008.03.003
Chatoui, K.; Talbaoui, A.; Aneb, M.; Bakri, Y.; Harhar, H.; Tabyaoui, M. Phytochemical screening, antioxidant and antibacterial activity of Lepidium sativum seeds from Morocco. Journal of Materials and Environmental Science 2016, 7(8): 2938-2946
Hussein, HM. Analysis of trace heavy metals and volatile chemical compounds of Lepidium sativum using atomic absorption spectroscopy, gas chromatography – mass spectrometric and fourier transform infrared spectroscopy. Research Journal of Pharmaceutical Biological and Chemical Sciences 2016, 7(4):2529-2555.
Sunil, C.; Kumar, V.; Van Staden, J. In vitro alpha-glucosidase inhibitory, total phenolic composition, antiradical and antioxidant potential of Heteromorpha arborescens (Spreng.) Cham. & Schltdl. leaf and bark extracts. South African Journal of Botany 2019, 124: 380-386. https://doi.org/10.1016/j.sajb.2019.05.017
Chatoui, K.; Harhar, H.; El Kamli, T.; Tabyaoui, M. Chemical Composition and Antioxidant Capacity of Lepidium sativum Seeds from Four Regions of Morocco. Evidence‐Based Complementary and Alternative Medicine 2020, 2020(1):7302727. https://doi.org/10.1155/2020/7302727
Omer, AB.; Nour AH.; Ali, MM.; Ishag, OA Omer.; Erwa, IY.; Ali, MA. Phytochemical screening, antimicrobial and antioxidant activity of Lepidium sativum seeds extract. South Asian Research Journal of Natural Products 2020, 3(1):10-17.
Yusof, WNSW.; Abdullah, H. Phytochemicals and cytotoxicity of Quercus infectoria ethyl acetate extracts on human cancer cells. Tropical Life Sciences Research 2020, 31(1): 69–84. https://doi.org/10.21315/tlsr2020.31.1.5
Ghebremariam, YS.; Demoz, MS.; Fissehaye, NA. Phytochemical Screening and Antimicrobial Potential of Lepidium sativium and Rumex nervosus in Eritrea. Journal of Advances in Medical and Pharmaceutical Sciences 2018, 19(1):1-8. https://doi.org/10.21315/tlsr2020.31.1.5
Système général harmonisé (SGH). (2013) : classification et étiquetage des produits chimiques. 5ème édition révisée. https://unece.org/fileadmin/DAM/trans/danger/publi/ghs/ghsrev05/French/ST-SG-AC10-30- Rev5f.pdf
Abuelgasim, AI.; Nuha, HS.; Mohammed, AH. Hepatoprotective effect of Lepidium sativum against carbon tetrachloride induced damage in rats. Research Journal of Animal and Veterinary Sciences 2008, 3: 20-23.
Wadhwa, S.; Panwar, MS.; Agrawal, A.; Saini, N.; Patidar, LPL. A Review on pharmacognostical study of lepidium sativum. Advance Research in Pharmaceuticals and biologicals 2012, 2(4): 316323.
Al-Asmari, AK.; Athar, MT.; Al-Shahrani, HM.; Al-Dakheel, SI.; Al-Ghamdi, MA. Efficacy of Lepidium sativum against carbon tetra chloride induced hepatotoxicity and determination of its bioactive compounds by GC- MS. Toxicology reports 2015, 2: 1319-1326. https://doi.org/10.1016/j.toxrep.2015.09.006
Gaamoune S. and Nouioua, W. In vitro Antioxidant and anti-inflammatory activities assessment of flavonoids crude extract of Anthemis pedunculata subsp. atlantica (Pomel) Oberpr., aerial parts. Algerian journal of Biosciences. 2023, 04(02): 96-101.
Mulondo, S.; Afaf , L. .; Hayet, C.; Muzamiru, K. Assessment of Marrubium vulgare hydro-alcoholic extract’s biological activities. Algerian journal of Biosciences 2023, 4(1): 1-8. Retrieved from https://www.journal.acse.science/index.php/ajb/article/view/129.
Ghadbane M., Bounar R., Rebbas K. Antimicrobial and antioxidant activity of methanol extract of Echinophora spinosa L. from Jijel, Algeria. Algerian Journal of Biosciences 2020, 1(1): 24-29. http://dx.doi.org/10.5281/zenodo.4076344.
Sakran, M.; Selim, Y.; Zidan, N. A new isoflavonoid from seeds of Lepidium sativum L. and its protective effect on hepatotoxicity induced by paracetamol in male rats. Molecules, 2014, 19(10): 15440-15451. https://doi.org/10.3390/molecules191015440.
Lee, CC.; Shen, SR.; Lai, YJ.; Wu, SC. Rutin and quercetin, bioactive compounds from tartary buckwheat, prevent liver inflammatory injury. Food & function 2013, 4(5): 794-802.
Kibiti, CM. and Afolayan, AJ. Preliminary phytochemical screening and biological activities of Bulbine abyssinica used in the folk medicine in the Eastern Cape Province, South Africa. Evidence‐based Complementary and Alternative Medicine 2015, 2015(1): 617607. https://doi.org/10.1039/c3fo30389f.
Al-Marzoqi, AH.; Al-Khafaji, NMS.; Hussein, HJ. In vitro antibacterial activity assessment of the crude phenolic, alkaloid and terpenoid compounds extracts of Lepidium sativum L. on human pathogenic bacteria. International Journal of ChemTech Research, 2016, 9(4) : 529-532. https://doi.org/10.1039/C3FO30389F.
Elouafy, Y.; El Yadini, A.; Mortada, S.; Hnini, M.; Harhar, H.; Khalid, A… et al. Antioxidant, antimicrobial, and α-glucosidase inhibitory activities of saponin extracts from walnut (Juglans regia L.) leaves. Asian Pacific Journal of Tropical Biomedicine 2023, 13(2) : 60-69. doi: 10.4103/2221-1691.369610.