Effect of gamma radiation on the antibacterial activity of Syzygium aromaticum

Samia Hadj Rabia; Yamina  Hadj Rabia; Fatima Benmoussa; Sarah Medjber; Ramy Nouri; Amine Mokhtari; Belkacem Mansouri

doi:10.57056/ajet.v8i2.115

Authors

Samia Hadj Rabia Department of Nuclear Applications, Nuclear Research Center of Draria, Algeria
Yamina Hadj Rabia Department of Cellular and Molecular Biology ,Faculty of Biological Science, University of Science and Technology, Houari Boumediene (USTHB), Algiers, Algeria;
Fatima Benmoussa Department of Nuclear Applications, Nuclear Research Center of Draria, Algeria
Sarah Medjber Department of Nuclear Applications, Nuclear Research Center of Draria, Algeria
Ramy Nouri Department of Nuclear Applications, Nuclear Research Center of Draria, Algeria
Amine Mokhtari Department of Nuclear Applications, Nuclear Research Center of Draria, Algeria
Belkacem Mansouri Department of operational exploitation and maintenance of the experimental platform, Nuclear Research Center of Algiers, Algeria

DOI:

https://doi.org/10.57056/ajet.v8i2.115

Keywords:

Syzygium aromaticum, polyphenols, flavonoids, antibacterial activity, Gamma radiation

Abstract

Irradiation is known as an effective method for decontamination and preservation of chemical and nutritional properties of various medicinal herbs and spices. This study was undertaken to evaluate the influence of irradiation dose (10kGy) on the antibacterial potency of Syzygium aromaticum (clove), plant known for its various biological properties notably its powerful antimicrobial power. Syzygium aromaticum (S. aromaticum) extract, prepared by maceration in methanol, was characterized by phytochemical analysis and colorimetric determination of chemical compounds. The antibacterial potential was also evaluated against this extract by observing growth inhibition zones. The result of gamma radiation effect on the clove, showed the preservation of main chemical constituents, with a significant increase in the content of total polyphenols and flavonoids. The methanolic extract of S. aromaticum revealed an inhibitory effect on the resistant strains, which improved significantly under the gamma radiation effect. These results seem very encouraging and suggest using gamma radiation as procedure to decontaminate the medicinal herbs and spices and improve the composition of extracts in bioactive molecules as well as their biological activities.

References

Mostafa AA, Al-askar A, Almaary KS, Dawoud TM, Sholkamy EN, Bakri MM. Antimicrobial activity of some plant extracts against bacterial strains causing food poisoning diseases. Saudi Journal of Biological Sciences. 2018, 25(2): 361-366.

Pereira E, Pimenta AI, Calhelha RC, Antonio AL, Barros L, Santos-Buelga C, Verde SC, Ferreira ICFR. Infusions of gamma irradiated Aloysia citrodora L. and Mentha x piperita L: Effects on phenolic composition, cytotoxicity, antibacterial and virucidal activities. Industrial Crops and Products. 2017, 97: 582-590.

Batiha G, Alkazmi L, Wasef L, Beshbishy AM, Nadwa EH, Rashwan EK. Syzygium aromaticum L.(Myrtaceae):Traditional Uses, Bioactive Chemical Constituents, Pharmacological and Toxicological Activities. Biomolécules. 2020,10(2):1-3.

Trease GE, Evans WC. A textbook of Pharmacogno.sy (13th edition) Bacilluere Tinal Ltd, London; 1989

Harbone JB. Methods of extraction and isolation in phytochemical methods, 3rd Edition, Chapman and Hall, London; 1998, p60-66.

Wang X, Sankarapandian K, Chen Y, Woo SO, Kwon HW, Perumalsamy H. & Ahn YJ. Relationship between total phenolic contents and biological properties of propolis from 20 different regions in South Korea. BMC Complementary and Alternative Medicine. 2016, 16(1): 1-12.

Gumus T, Albayrak S, Sagdic O, Arici M. Effet de l'irradiation gamma sur le contenu phénolique total et les activités antioxydantes de Satureja Hortensis, Thymus Vulgaris et Thymbra Spicata de Turquie. International Journal of Food Properties. 2011, 14(4) : 830-839.

Abolhasani A, Barzegar M, Sahari MA. Effect of gamma irradiation on the extraction yield, antioxidant, and antityrosinase activities of pistachio green hull extract. Radiation Physics and Chemistry. 2017, 144 : 373-378.

Altıok E, Bayçın D, Bayraktar O, Ülkü S. Isolement de polyphénols à partir d'extraits de feuilles d'olivier (Olea europaea L.) par adsorption sur fibroïne de soie. Technologie de séparation et de purification. 2008, 62(2): 342-348.

Gaspar EM, Santana JC, Santos PM, Telo JP, Vieira AJ. Gamma irradiation of clove: level of trapped radicals and effects on bioactive composition. Journal of the Science of Food and Agriculture. 2019, 99(4):1668-1674.

El-Beltagi HS, Dhawi F, Aly AA, El Ansary AE. Chemical compositions and biological activities of Chemical compositions and biological activities of the essential oils the essential oils from gamma irradiated celery (Apium graveolens L.) seeds. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2020, 48(4): 2114-2133.

Abdelaleem MA, Elbassiony RAK. Evaluation of phytochemicals and antioxidant activity of gamma irradiated quinoa (Chenopodium quinoa). Brazilian Journal of Biology. 2021, 81(3): 806-813.

Variyar PS. Effect of gamma-irradiation on the phenolic acids of some Indian spices. International Journal Food Science Technology. 1998, 33: 533-537.

Suhaj M, Horvathova J. Changes in antioxidant activity induced by irradiation of clove (Syzygium aromaticum) and ginger (Zingiber officinale). Journal of Food and Nutrition Research. 2007, 46(3): 112-122.

Adamo M, Capitani D, Mannina L, Cristinzio M, Ragni P, Tata A, Coppola R. Truffles decontamination treatment by ionizing radiation. Radiation Physics and Chemistry. 2004, 71(1-2):167-170.

Abdeldaim GM, Strålin K, Kirsebom LA, Olcén P, Blomberg J, Herrmann B. Detection of Haemophilus influenzae in respiratory secretions from pneumonia patients by quantitative real-time polymerase chain reaction. Diagnostic Microbiology And Infectious Disease. 2009, 64(4): 366-373.

Vardhan PV, Shukla LI. Gamma irradiation of medicinally important plants and the enhancement of secondary metabolite production. International Journal of Radiation Biology. 2017, 93(9):967-979.

Mohamed LK, Sulieman MA, Yagoub AEA, Mohammed MA, Alhuthayli HF, Mohamed IA, Almaiman SA, Alfawaz MA, Osman MA, Hassan AB. Changes in Phytochemical Compounds and Antioxidant Activity of Two Irradiated Sorghum (Sorghum bicolor (L.) Monech) Cultivars during the Fermentation and Cooking of Traditional Sudanese Asida. Fermentation. 2022, 8(2): 1-10.

Khattak G, Ashraf M, Khan M. Assessment of genetic variation for yield and yield components in mungbean (Vigna radiate (L.) Wilczek) using generation mean analysis. Pakistan Journal of Botany. 2004, 36(3): 583-588.

Eamsiri J, Chookaew S, Pewlong W, Sajjabut S, Orpong P. Effects of irradiation on antioxidant and antimicrobial activities of Coscinium fenestratum (Goetgh.) Colebr. In Journal of Physics: Conference Series. 2019, 1285(2019) 012006.

Khattak KF, Simpson TJ, Ihasnullah. Effect of gamma irradiation on the extraction yield, total phenolic content and free radical-scavenging activity of Nigella staiva seed. Food Chemistry. 2008, 110(4): 967-972.

Pei R, Zhou F, Ji B, Xu J. Evaluation of Combined Antibacterial Effects of Eugenol, Cinnamaldehyde, Thymol, and Carvacrol against with an improved Method. Institute of Food Technologists. 2009, 74(7): 379-383.